【分析】基于荧光杂交探针的细胞内NRAS RNA G-四链体结构的精确检测
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><a style="color: black;"><span style="color: black;"><img src="http://mmbiz.qpic.cn/mmbiz_gif/hUDFo5ricnjXTzvjPF35RnRqSFWcBf5w0emVsfvpaibnLUQqTwSxJxKxO0voicdsKZg2JzyuVCqn7zt6KsGV2iaE6A/0?wx_fmt=gif&tp=webp&wxfrom=5&wx_lazy=1" style="width: 50%; margin-bottom: 20px;"></span></a></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;">RNA多样的生物学功能与其多变的拓扑结构密切<span style="color: black;">关联</span>。无论在细胞外还是在细胞内，对RNA二级结构的检测都是<span style="color: black;">科研</span>的热点。由富鸟嘌呤（G）序列形成的G-四链体结构是其中一种重要的功能性RNA二级结构。<span style="color: black;">日前</span>，<span style="color: black;">科研</span>人员<span style="color: black;">能够</span><span style="color: black;">运用</span>小分子探针<span style="color: black;">或</span>特异性抗体对细胞内整体的RNA G-四链体结构进行检测。<span style="color: black;">然则</span>，转录组中存在众多的富G序列。由<span style="color: black;">区别</span>富G序列所形成的G-四链体，它们结构<span style="color: black;">类似</span>，但存在、功能与相互<span style="color: black;">功效</span>对象却大不相同。<span style="color: black;">怎样</span>在数量庞大的其他RNA富G序列干扰下，特异性对单一指定富G序列所形成的G-四链体结构进行检测是一大挑战。</span></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;"><span style="color: black;">近期</span>，<strong style="color: blue;">中山大学药学院</strong>的<span style="color: black;"><strong style="color: blue;">谭嘉恒副教授</strong></span>与<span style="color: black;"><strong style="color: blue;">黄志纾教授</strong></span><strong style="color: blue;">课题组</strong>首次<span style="color: black;">报告</span>了<strong style="color: blue;">基于杂交技术</strong>所设计的<strong style="color: blue;">新型G-四链体荧光探针</strong>——GTFH（G-Quadruplex-Triggered Fluorogenic Hybridization）探针。该类探针由两部分<span style="color: black;">构成</span>，一部分为特异性的小分子G-四链体荧光探针，而另一部分则是能与富G RNA邻近序列进行杂交的DNA分子，两者<span style="color: black;">经过</span>点击化学偶联。<span style="color: black;">科研</span>人员以NRAS mRNA的 5′-UTR富G序列为靶标，设计合<span style="color: black;">成为了</span>能<span style="color: black;">选定</span>对该序列所形成的G-四链体结构进行检测的GTFH探针ISCH-nras1。</span></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><img src="http://mmbiz.qpic.cn/mmbiz_png/hUDFo5ricnjXBTqJdhjOnQdicZ9yqLaKic6C3icg3QrbDg7Jhrg6ibDnibatuBsdYqTfmbibicqJeCweibUWC0ZQCHIbMBw/640?wx_fmt=png&tp=webp&wxfrom=5&wx_lazy=1&wx_co=1" style="width: 50%; margin-bottom: 20px;"></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;">应用探针ISCH-nras1，<span style="color: black;">科研</span>人员实现了细胞内NRAS mRNA 5′-UTR中G-四链体结构的<span style="color: black;">有效</span>精确检测。GTFH探针设计合成简便，<span style="color: black;">能够</span>在其他G-四链体结构的检测中推广应用。</span></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;">该<span style="color: black;">科研</span>成果在《<strong style="color: blue;">美国化学学会会刊</strong>》（<strong style="color: blue;">Journal of the American Chemical Society</strong>）上<span style="color: black;">发布</span>，<strong style="color: blue;"><span style="color: black;">第1</span>作者</strong>是<span style="color: black;"><strong style="color: blue;">陈硕斌博士</strong></span>。</span></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;">该<span style="color: black;">科研</span>工作得到了国家自然科学基金和广东省自然科学杰出青年基金的大力支持。</span></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;">http://pubs.acs.org/doi/abs/10.1021/jacs.6b04799</p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;">原文：Visualization of NRAS RNA G-Quadruplex Structures in Cells with an Engineered Fluorogenic Hybridization Probe</p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;">J. Am. Chem. Soc., <strong style="color: blue;">2016</strong>, 138, 10382-10385, DOI: 10.1021/jacs.6b04799</p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><strong style="color: blue;"><strong style="color: blue;"><strong style="color: blue;"><strong style="color: blue;"><span style="color: black;"><strong style="color: blue;"><span style="color: black;"><strong style="color: blue;"><span style="color: black;"><strong style="color: blue;"><strong style="color: blue;"><strong style="color: blue;"><span style="color: black;"><span style="color: black;">X-MOL</span><span style="color: black;">分析<span style="color: black;">行业</span></span><span style="color: black;">学术讨论QQ群（</span></span><span style="color: black;">292125992</span><span style="color: black;">）</span></strong></strong></strong></span></strong></span></strong></span></strong></strong></strong></strong></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;">举荐</span>阅读</p><a style="color: black;">2016上半年全国高校<span style="color: black;">科研</span>所在“四大”化学期刊上论文<span style="color: black;">发布</span>统计</a><a style="color: black;">在手机上<span style="color: black;">运用</span>“文献直达”是一种什么样的体验？</a><a style="color: black;">不是APP，胜似APP，快来将X-MOL移动版添加</a>至桌面！<a style="color: black;">先定一个小<span style="color: black;">目的</span>，<span style="color: black;">例如</span>免费给自己简历润个色？</a>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;">本文版权属于</span><span style="color: black;"><strong style="color: blue;">X-MOL</strong></span><span style="color: black;">（x-mol.com），未经许可谢绝转载！欢迎读者<span style="color: black;">伴侣</span>们分享到<span style="color: black;">伴侣</span>圈or<span style="color: black;">博客</span>！</span></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;"><strong style="color: blue;">长按</strong></span>下图<strong style="color: blue;">识别图中二维码</strong>，<span style="color: black;">容易</span>关注<span style="color: black;">咱们</span>！</p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><img src="http://mmbiz.qpic.cn/mmbiz/hUDFo5ricnjWDTWoAicacJ3BWzftIziahPMfqfqbUV8qlAybFq0g2f3uVhLAmVCdIk6GaPWswaNzp7rojfJia7cQ6w/640?wx_fmt=jpeg&tp=webp&wxfrom=5&wx_lazy=1&wx_co=1" style="width: 50%; margin-bottom: 20px;"></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><img src="http://mmbiz.qpic.cn/mmbiz/hUDFo5ricnjWPHkAKnW2eeSQzbBib5Eo8qTa11daufTE7eLHLSN4YF3icZbaqOlk23LxpOvTaMNfVENicmyE4DibdpQ/0?wx_fmt=gif&tp=webp&wxfrom=5&wx_lazy=1" style="width: 50%; margin-bottom: 20px;"></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;">点击“<span style="color: black;">阅读原文</span>”，参加讨论</span></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;"><img src="http://mmbiz.qpic.cn/mmbiz/hUDFo5ricnjWPHkAKnW2eeSQzbBib5Eo8ql4xJay2BqYq9QrV3fQ2uDibu5qzN0rYfSicDxmgtC8jFJtMBy2ib90yDg/0?wx_fmt=gif&tp=webp&wxfrom=5&wx_lazy=1" style="width: 50%; margin-bottom: 20px;"></span></p>
期待你更多的精彩评论，一起交流学习。 这夸赞甜到心里，让我感觉温暖无比。 楼主听话，多发外链好处多，快到碗里来！外链论坛 http://www.fok120.com/
页:
[1]