9q13nh 发表于 2024-10-10 09:45:40

核素PET探针 Ga-HZ20靶向rhACE2的Micro-PET分子影像的科研


    <p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;">&nbsp; &nbsp; 2023<span style="color: black;">年</span><span style="color: black;">11</span><span style="color: black;">月，北京大学肿瘤医院</span></span><span style="color: black;">、</span><span style="color: black;">北京大学深圳<span style="color: black;">科研</span>生院</span><span style="color: black;">及</span><span style="color: black;">西南医科大学<span style="color: black;">基本</span>医学院</span><span style="color: black;">，</span><span style="color: black;">合作在</span><span style="color: black;">Wiley<span style="color: black;">出版社（</span></span><span style="color: black;"><span style="color: black;">医学</span>-<span style="color: black;">病毒学</span></span><span style="color: black;">国际一流刊物）</span><strong style="color: blue;"><span style="color: black;">《医学病毒学杂志》</span></strong><span style="color: black;">JOURNAL OF MEDICAL VIROLOGY<span style="color: black;">（</span><span style="color: black;">JCR Q1</span><span style="color: black;">）正式<span style="color: black;">发布</span>了题为：</span><span style="color: black;">“Molecular PET/CT mapping of rhACE2 distribution and quantification in organs to aid in SARS‐CoV‐2 targeted therapy”</span><span style="color: black;">的<span style="color: black;">科研</span>论文。</span></span></p>
    <p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><img src="https://mmbiz.qpic.cn/sz_mmbiz_png/Sdlx2O7ibpVnjQ4splicZicMhQuTuSWJ8KUwWB0DaEUjFBCWOnwfiaibLb5KtwlFgrsKnrYxzibJt6y8Pfmt2snAvJRA/640?wx_fmt=png&amp;from=appmsg&amp;tp=webp&amp;wxfrom=5&amp;wx_lazy=1&amp;wx_co=1" style="width: 50%; margin-bottom: 20px;"></p>
    <p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;"><span style="color: black;">&nbsp; &nbsp; 团队依托<span style="color: black;">安身</span>于</span>SARS-CoV-2<span style="color: black;">的<span style="color: black;">需要</span>，<span style="color: black;">研发</span>了基于血管紧张素转化酶</span><span style="color: black;">2</span><span style="color: black;">（</span><span style="color: black;">ACE2</span><span style="color: black;">）</span></span><span style="color: black;"><span style="color: black;">的</span></span><span style="color: black;"><span style="color: black;">核素探针，并在</span>rhACE2<span style="color: black;">组织</span></span><span style="color: black;">/</span><span style="color: black;"><span style="color: black;">器官原位模型中进行</span></span><span style="color: black;"><span style="color: black;">的分子影像可视化</span></span><span style="color: black;"><span style="color: black;"><span style="color: black;">评定</span></span></span><span style="color: black;"><span style="color: black;"><span style="color: black;">科研</span></span></span><span style="color: black;"><span style="color: black;">。</span></span></p>
    <p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;"><strong style="color: blue;"><span style="color: black;"><span style="color: black;">科研</span>背景：</span></strong></span></p>
    <p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;"><span style="color: black;">&nbsp; &nbsp; 血管紧张素转换酶</span>2 (ACE2)<span style="color: black;">是</span><span style="color: black;">SARS‐CoV‐2</span><span style="color: black;">感染人体的关键受体，其在肾素</span><span style="color: black;">‐</span><span style="color: black;">血管紧张素系统中起关键负调控<span style="color: black;">功效</span>，并在正常生理<span style="color: black;">要求</span>下促进肾脏和肠道中的氨基酸运输。<span style="color: black;">科研</span><span style="color: black;">发掘</span>，</span><span style="color: black;">ACE2</span></span><span style="color: black;">可能<span style="color: black;">作为</span></span><span style="color: black;"><span style="color: black;">较好的治疗靶点，<span style="color: black;">由于</span></span></span><strong style="color: blue;"><span style="color: black;"><span style="color: black;">无论</span>SARS-CoV-2<span style="color: black;"><span style="color: black;">怎样</span>进化，都依赖于</span><span style="color: black;">ACE2</span><span style="color: black;">进入人体细胞</span></span></strong><span style="color: black;"><span style="color: black;">。</span></span></p>
    <p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;"><span style="color: black;">&nbsp; &nbsp; 重组人</span>ACE2 (rhACE2)<span style="color: black;"><span style="color: black;">做为</span>该蛋白的可溶性形式，<span style="color: black;">做为</span>诱饵，<span style="color: black;">能够</span>阻断病毒与机体膜结合形式的</span><span style="color: black;">ACE2</span><span style="color: black;">结合。<span style="color: black;">日前</span>多项临床前及临床<span style="color: black;">实验</span><span style="color: black;">科研</span><span style="color: black;">显示</span>，</span><span style="color: black;">rhACE2</span><span style="color: black;"><span style="color: black;">能够</span>有效中和病毒、预防感染及缓解病情。<span style="color: black;">另外</span>，</span><span style="color: black;">rhACE2</span><span style="color: black;"><span style="color: black;">做为</span>外源性<span style="color: black;">弥补</span>物，可缓解因病毒感染后</span><span style="color: black;">ACE2</span><span style="color: black;">下降而<span style="color: black;">引起</span>的</span><span style="color: black;">RAS</span><span style="color: black;">系统失衡。然而，<span style="color: black;">因为</span></span><span style="color: black;">ACE2</span><span style="color: black;">在</span><span style="color: black;">RAS</span><span style="color: black;">系统中的负调控<span style="color: black;">功效</span>，<span style="color: black;">针对</span>某些有合并症的<span style="color: black;">病人</span><span style="color: black;">运用</span></span><span style="color: black;">rhACE2</span><span style="color: black;">时应更加<span style="color: black;">小心</span>。本<span style="color: black;">科研</span>首次成功构建了</span><span style="color: black;">rhACE2-</span><span style="color: black;">组织</span><span style="color: black;">/</span><span style="color: black;">器官原位模型，<span style="color: black;">创立</span>了</span><span style="color: black;">rhACE2</span><span style="color: black;">给药剂量与分子影像的高度<span style="color: black;">关联</span>性。<span style="color: black;">经过</span></span><span style="color: black;">ACE2</span><span style="color: black;">特异性核素探针</span></span><span style="color: black;">68</span><span style="color: black;">Ga-HZ20<span style="color: black;">动态监测组织</span><span style="color: black;">/</span><span style="color: black;">器官原位的</span><span style="color: black;">rhACE2</span><span style="color: black;">，有助于<span style="color: black;">评定</span></span><span style="color: black;">rhACE2 </span><span style="color: black;">在器官中的驻留能力、分析其对 </span><span style="color: black;">SARS-CoV-2 </span><span style="color: black;">的预防能力和对</span><span style="color: black;">COVID-19 </span><span style="color: black;">的治疗<span style="color: black;">潜能</span>，<span style="color: black;">同期</span><span style="color: black;">亦</span>为</span><span style="color: black;">rhACE2</span><span style="color: black;">疗法<span style="color: black;">精细</span><span style="color: black;">方法</span>的制定<span style="color: black;">供给</span>了新的见解和思路。</span></span></p>
    <p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><strong style="color: blue;"><span style="color: black;"><span style="color: black;"><span style="color: black;">科研</span><span style="color: black;">办法</span>：</span></span></strong></p>
    <p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;"><span style="color: black;">&nbsp; &nbsp; 本<span style="color: black;">科研</span>在课题组前期工作<span style="color: black;">基本</span>上，<span style="color: black;">经过</span><span style="color: black;">调节</span>标记温度，<span style="color: black;">加强</span>了探针的放射化学产率和比活度。采用放射性</span>-<span style="color: black;"><span style="color: black;">有效</span>液相色谱法（</span>Radio-HPLC<span style="color: black;">）对探针的标记率和稳定性进行</span></span><span style="color: black;"><span style="color: black;">检测</span></span><span style="color: black;"><span style="color: black;">。给小鼠注射高剂量的探针后采用</span>HE<span style="color: black;">染色<span style="color: black;">评定</span>探针的安全性，并<span style="color: black;">运用</span>免疫组织化学染色（</span>IHC<span style="color: black;">）检测小鼠<span style="color: black;">重点</span>脏器中</span>ACE2<span style="color: black;">的本底表达水平。在对探针进行综合表征后，构建</span>rhACE2<span style="color: black;">在小鼠皮下、肿瘤、肝脏、脾脏和脑的原位模型，并<span style="color: black;">经过</span></span>Micro-PET/ CT<span style="color: black;">示踪</span>rhACE2<span style="color: black;">的分布， </span></span><span style="color: black;"><span style="color: black;"><span style="color: black;">经过</span></span></span><span style="color: black;">SUV<span style="color: black;">值、靶本比（</span>rhACE2<span style="color: black;">注射部位与肌肉）、靶部位的离体显像及</span>% ID/g<span style="color: black;">比较<span style="color: black;">区别</span>剂量组之间的探针摄取差异。最后，分析靶部位探针摄取与</span>rhACE2<span style="color: black;">给药剂量的<span style="color: black;">关联</span>性，并<span style="color: black;">经过</span></span>Western blot<span style="color: black;">对模型进行验证。</span></span></p>
    <p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;"><strong style="color: blue;"><span style="color: black;"><span style="color: black;">科研</span>结果：</span></strong></span></p>
    <p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><strong style="color: blue;"><span style="color: black;">1.</span><span style="color: black;">68</span><span style="color: black;">Ga‐HZ20<span style="color: black;">探针的标记、体外特、安全性<span style="color: black;">评估</span>及</span>ACE2<span style="color: black;">在小鼠器官中的表达</span></span></strong></p>
    <p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;">&nbsp; &nbsp; 68</span><span style="color: black;">Ga‐HZ20<span style="color: black;">放射性合成过程如图</span><span style="color: black;">1A</span><span style="color: black;">所示。在</span><span style="color: black;">65°C</span><span style="color: black;">、</span><span style="color: black;">85°C</span><span style="color: black;">和</span><span style="color: black;">95°C</span><span style="color: black;"><span style="color: black;">要求</span>下，</span></span><span style="color: black;">68</span><span style="color: black;">Ga‐HZ20<span style="color: black;">探针的放射性标记率分别为</span><span style="color: black;">74.03%</span><span style="color: black;">、</span><span style="color: black;">96.42%</span><span style="color: black;">和</span><span style="color: black;">78.60%(</span><span style="color: black;">图</span><span style="color: black;">1B)</span><span style="color: black;">，放射化学产率分别为</span><span style="color: black;">61.51±4.47%</span><span style="color: black;">、</span><span style="color: black;">78.99±3.92%</span><span style="color: black;">和</span><span style="color: black;">66.34±5.51% (</span><span style="color: black;">图</span><span style="color: black;">1C)</span><span style="color: black;">。</span></span><span style="color: black;">68</span><span style="color: black;">Ga - HZ20<span style="color: black;">在常温下</span><span style="color: black;">180 min</span><span style="color: black;">内，放射化学纯度均高于</span><span style="color: black;">96% (</span><span style="color: black;">图</span><span style="color: black;">1D)</span><span style="color: black;">。<span style="color: black;">同期</span>高剂量的探针并未对小鼠<span style="color: black;">导致</span>器质性<span style="color: black;">损害</span></span><span style="color: black;">(</span><span style="color: black;">图</span><span style="color: black;">1E)</span><span style="color: black;">。在小鼠支气管上皮细胞、肾脏和小肠中均观察到</span><span style="color: black;">ACE2</span><span style="color: black;">高表达</span><span style="color: black;">(++)(</span><span style="color: black;">图</span><span style="color: black;">1F)</span><span style="color: black;">，该结果支持</span><span style="color: black;">SARS-CoV-2</span><span style="color: black;">的<span style="color: black;">呼气</span>道和粪</span><span style="color: black;">-</span><span style="color: black;">口传播，以及</span><span style="color: black;">SARS-CoV-2</span><span style="color: black;">在感染后<span style="color: black;">导致</span>肾<span style="color: black;">损害</span>和腹泻等。</span></span></p>
    <p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><img src="https://mmbiz.qpic.cn/sz_mmbiz_png/Sdlx2O7ibpVnjQ4splicZicMhQuTuSWJ8KUiaUsAIQfQ6BT9kEicgVPKBIsFic7a3dRzPoPjh0pR5UfBn70q14XLmibEg/640?wx_fmt=png&amp;from=appmsg&amp;tp=webp&amp;wxfrom=5&amp;wx_lazy=1&amp;wx_co=1" style="width: 50%; margin-bottom: 20px;"></p>
    <p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;"><span style="color: black;">图</span>1. (A) </span><span style="color: black;">68</span><span style="color: black;">Ga‐HZ20<span style="color: black;">探针的合成示意图。</span><span style="color: black;">(B) </span></span><span style="color: black;">68</span><span style="color: black;">Ga‐HZ20<span style="color: black;">在<span style="color: black;">区别</span>温度下的放射性标记率。</span><span style="color: black;">(C)</span></span><span style="color: black;">&nbsp;68</span><span style="color: black;">Ga‐HZ20<span style="color: black;">在<span style="color: black;">区别</span>温度下的放射化学产率。</span><span style="color: black;">(D) </span></span><span style="color: black;">68</span><span style="color: black;">Ga‐HZ20<span style="color: black;">在生理盐水和</span><span style="color: black;">5% HSA</span><span style="color: black;">中的稳定性。</span><span style="color: black;">(E)</span><span style="color: black;">小鼠<span style="color: black;">重点</span>器官</span><span style="color: black;">HE</span><span style="color: black;">染色。</span><span style="color: black;">(F)</span><span style="color: black;">小鼠<span style="color: black;">重点</span>器官中</span><span style="color: black;">ACE2</span><span style="color: black;">表达水平的</span><span style="color: black;">IHC</span><span style="color: black;">染色。</span></span></p>
    <p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><strong style="color: blue;"><span style="color: black;">2<span style="color: black;">．</span></span><span style="color: black;">68</span><span style="color: black;">Ga-HZ20<span style="color: black;">在</span><span style="color: black;">rhACE2</span><span style="color: black;">皮下模型中的显像<span style="color: black;">科研</span></span></span></strong></p>
    <p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;"><span style="color: black;">&nbsp; &nbsp; 向</span>KM<span style="color: black;">小鼠右肩分别注射<span style="color: black;">区别</span>剂量</span><span style="color: black;">(0.1</span><span style="color: black;">、</span><span style="color: black;">0.2</span><span style="color: black;">、</span><span style="color: black;">0.4</span><span style="color: black;">、</span><span style="color: black;">0.8 nmol)</span><span style="color: black;">的</span><span style="color: black;">rhACE2</span><span style="color: black;">蛋白。</span><span style="color: black;">PET</span><span style="color: black;"><span style="color: black;">影像</span><span style="color: black;">表示</span>，</span></span><span style="color: black;">68</span><span style="color: black;">Ga‐HZ20<span style="color: black;">在<span style="color: black;">身体</span>对</span><span style="color: black;">rhACE2</span><span style="color: black;"><span style="color: black;">拥有</span>很高的特异性和<span style="color: black;">敏锐</span>性</span><span style="color: black;">(</span><span style="color: black;">图</span><span style="color: black;">2A)</span><span style="color: black;">，该探针<span style="color: black;">能够</span>准确定位</span><span style="color: black;">rhACE2</span><span style="color: black;">聚集的区域，揭示</span><span style="color: black;">rhACE2</span><span style="color: black;">的分布。并且摄取随着</span><span style="color: black;">rhACE2</span><span style="color: black;">注射剂量的<span style="color: black;">增多</span>而<span style="color: black;">增多</span>。探针摄取在</span><span style="color: black;">120 min</span><span style="color: black;">达到峰值，<span style="color: black;">而后</span>缓慢下降</span><span style="color: black;">(</span><span style="color: black;">图</span><span style="color: black;">2C)</span><span style="color: black;">。随着</span><span style="color: black;">rhACE2</span><span style="color: black;">注射量的<span style="color: black;">增多</span>，探针的靶本比<span style="color: black;">亦</span>随之<span style="color: black;">增多</span>，在</span><span style="color: black;">240 min</span><span style="color: black;">时各剂量组分别达到</span><span style="color: black;">2.50±0.41</span><span style="color: black;">、</span><span style="color: black;">8.24±0.47</span><span style="color: black;">、</span><span style="color: black;">15.57±0.49</span><span style="color: black;">和</span><span style="color: black;">25.45±1.48(</span><span style="color: black;">图</span><span style="color: black;">2D)</span><span style="color: black;">。</span></span><span style="color: black;"><span style="color: black;">在体</span></span><span style="color: black;"><span style="color: black;">图像采集完成后，对</span>rhACE2 (0.2 nmol, 0.4 nmol)<span style="color: black;">注射部位和<span style="color: black;">重点</span>器官取样进行离体<span style="color: black;">影像</span></span><span style="color: black;">(</span><span style="color: black;">图</span><span style="color: black;">2E)</span><span style="color: black;">，结果<span style="color: black;">表示</span>探针在</span><span style="color: black;">rhACE2</span><span style="color: black;">注射部位有<span style="color: black;">显著</span>的剂量依赖性摄取。分析注射后</span><span style="color: black;">120 min SUVmax</span><span style="color: black;">与</span><span style="color: black;">rhACE2</span><span style="color: black;">剂量的<span style="color: black;">关联</span>性，</span><span style="color: black;">R</span></span><span style="color: black;">2</span><span style="color: black;"><span style="color: black;">为</span>0.9914(<span style="color: black;">图</span><span style="color: black;">2F)</span><span style="color: black;">。对</span><span style="color: black;">0.4 nmol</span><span style="color: black;">组进行阻断<span style="color: black;">科研</span>，观察到阻断组在</span><span style="color: black;">rhACE2</span><span style="color: black;">注射部位的摄取<span style="color: black;">显著</span>减少</span><span style="color: black;">(</span><span style="color: black;">图</span><span style="color: black;">2B)</span><span style="color: black;">，且在所有时间点，两组之间均存在<span style="color: black;">明显</span>性差异</span><span style="color: black;">(</span><span style="color: black;">图</span><span style="color: black;">2G)</span><span style="color: black;">。</span><span style="color: black;">Western blotting</span><span style="color: black;"><span style="color: black;">表示</span></span><span style="color: black;">rhACE2</span><span style="color: black;">蛋白被成功定<span style="color: black;">位置于</span>皮下注射部位，在<span style="color: black;">身体</span>保持稳定</span><span style="color: black;">(</span><span style="color: black;">图</span><span style="color: black;">2H)</span><span style="color: black;">。</span></span></p>
    <p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><img src="https://mmbiz.qpic.cn/sz_mmbiz_png/Sdlx2O7ibpVnjQ4splicZicMhQuTuSWJ8KUgtj2qXWvhaIJ7Joqdao5yp9icxMibAdydBERQa6vfiaaPHWsfSCZEia0icw/640?wx_fmt=png&amp;from=appmsg&amp;tp=webp&amp;wxfrom=5&amp;wx_lazy=1&amp;wx_co=1" style="width: 50%; margin-bottom: 20px;"></p>
    <p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;"><span style="color: black;">图</span>2. (A)<span style="color: black;">皮下注射<span style="color: black;">区别</span>剂量</span><span style="color: black;">rhACE2</span><span style="color: black;">后的</span><span style="color: black;">PET/CT</span><span style="color: black;"><span style="color: black;">影像</span>。白色圆圈为</span><span style="color: black;">rhACE2</span><span style="color: black;">注射部位。</span><span style="color: black;">(K, B</span><span style="color: black;"><span style="color: black;">表率</span>肾和膀胱</span><span style="color: black;">)</span><span style="color: black;">。</span><span style="color: black;">(B) 50 μg HZ20</span><span style="color: black;">阻断后小鼠的</span><span style="color: black;">PET</span><span style="color: black;"><span style="color: black;">影像</span>。</span><span style="color: black;">(C) rhACE2</span><span style="color: black;">注射部位</span><span style="color: black;">SUVmax</span><span style="color: black;">的比较。</span><span style="color: black;">(D) rhACE2</span><span style="color: black;">注射部位与肌肉的</span><span style="color: black;">SUVmax</span><span style="color: black;">比值。</span><span style="color: black;">(E)</span><span style="color: black;">注射部位和<span style="color: black;">重点</span>器官的离体<span style="color: black;">影像</span>。</span><span style="color: black;">(F) SUVmax</span><span style="color: black;">与</span><span style="color: black;">rhACE2</span><span style="color: black;">剂量<span style="color: black;">关联</span>性分析。</span><span style="color: black;">(G) 0.4 nmol</span><span style="color: black;">阻断与非阻断组的</span><span style="color: black;">SUVmax</span><span style="color: black;">比较。</span><span style="color: black;">(H)</span><span style="color: black;">注射部位</span><span style="color: black;">rhACE2</span><span style="color: black;">蛋白的</span><span style="color: black;">Western blot</span><span style="color: black;">。</span></span></p>
    <p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><strong style="color: blue;"><span style="color: black;">3<span style="color: black;">．</span></span><span style="color: black;">68</span><span style="color: black;">Ga-HZ20<span style="color: black;">在</span><span style="color: black;">rhACE2</span><span style="color: black;">肿瘤模型中的显像<span style="color: black;">科研</span></span></span></strong></p>
    <p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;"><span style="color: black;">在</span>A549<span style="color: black;">荷瘤小鼠瘤内注射<span style="color: black;">区别</span>剂量</span><span style="color: black;">rhACE2</span><span style="color: black;">后进行</span><span style="color: black;">PET/CT</span><span style="color: black;"><span style="color: black;">影像</span>，结果<span style="color: black;">表示</span>在</span><span style="color: black;">A549</span><span style="color: black;">肿瘤中未观察到探针的摄取；在</span><span style="color: black;">rhACE2</span><span style="color: black;">注射组肿瘤中的摄取随着剂量的<span style="color: black;">增多</span>而<span style="color: black;">增多</span></span><span style="color: black;">(</span><span style="color: black;">图</span><span style="color: black;">3A)</span><span style="color: black;">，反应出</span></span><span style="color: black;">68</span><span style="color: black;">Ga‐HZ20<span style="color: black;">能准确地示踪肿瘤中的</span><span style="color: black;">rhACE2</span><span style="color: black;">。在探针注射后</span><span style="color: black;">30 min</span><span style="color: black;">，肿瘤部位的</span><span style="color: black;">SUVmax</span><span style="color: black;">分别达到</span><span style="color: black;">0.3±0.08</span><span style="color: black;">、</span><span style="color: black;">0.6±0.07</span><span style="color: black;">、</span><span style="color: black;">0.75±0.03</span><span style="color: black;">和</span><span style="color: black;">0.94±0.03(</span><span style="color: black;">图</span><span style="color: black;">3B)</span><span style="color: black;">。对肿瘤与肌肉的</span><span style="color: black;">SUVmax</span><span style="color: black;">比值（靶本比）分析<span style="color: black;">表示</span>，在注射相同剂量的</span><span style="color: black;">rhACE2</span><span style="color: black;">蛋白时，该比值随时间而<span style="color: black;">增多</span>， </span><span style="color: black;">240 min</span><span style="color: black;">时靶本比分别为</span><span style="color: black;">0.88±0.54</span><span style="color: black;">、</span><span style="color: black;">4.59±0.32</span><span style="color: black;">、</span><span style="color: black;">6.22±1.56</span><span style="color: black;">、</span><span style="color: black;">7.13±0.54(</span><span style="color: black;">图</span><span style="color: black;">3C)</span><span style="color: black;">。肿瘤组织的离体<span style="color: black;">影像</span><span style="color: black;">亦</span>呈剂量依赖性</span><span style="color: black;">(</span><span style="color: black;">图</span><span style="color: black;">3D)</span><span style="color: black;">。</span><span style="color: black;">γ‐</span><span style="color: black;">计数分析<span style="color: black;">表示</span>，<span style="color: black;">区别</span>剂量组差异<span style="color: black;">明显</span></span><span style="color: black;">(</span><span style="color: black;">图</span><span style="color: black;">3E)</span><span style="color: black;">。探针注射后</span><span style="color: black;">30 min rhACE2</span><span style="color: black;">与</span><span style="color: black;">SUVmax</span><span style="color: black;">的<span style="color: black;">关联</span>性最高，</span><span style="color: black;">R</span></span><span style="color: black;">2</span><span style="color: black;"><span style="color: black;">为</span>0.9176(<span style="color: black;">图</span><span style="color: black;">3F)</span><span style="color: black;">。</span><span style="color: black;">Western blotting</span><span style="color: black;">进一步验证了显像结果</span><span style="color: black;">(</span><span style="color: black;">图</span><span style="color: black;">3G)</span><span style="color: black;">。</span></span></p>
    <p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><img src="https://mmbiz.qpic.cn/sz_mmbiz_png/Sdlx2O7ibpVnjQ4splicZicMhQuTuSWJ8KUKIaTrflQZneiaUpxIucDT0EWAIoTGXVtxgAUo8nXGOgBOIkZZ57K33w/640?wx_fmt=png&amp;from=appmsg&amp;tp=webp&amp;wxfrom=5&amp;wx_lazy=1&amp;wx_co=1" style="width: 50%; margin-bottom: 20px;"></p>
    <p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;"><span style="color: black;">图</span>3. (A)<span style="color: black;">瘤内注射<span style="color: black;">区别</span>剂量</span><span style="color: black;">rhACE2</span><span style="color: black;">后的</span><span style="color: black;">PET/CT</span><span style="color: black;"><span style="color: black;">影像</span>。带虚线的白色方框<span style="color: black;">暗示</span>肿瘤及</span><span style="color: black;">rhACE2</span><span style="color: black;">注射部位</span><span style="color: black;">(K, B</span><span style="color: black;">分别<span style="color: black;">表率</span>肾脏和膀胱</span><span style="color: black;">)</span><span style="color: black;">。</span><span style="color: black;">(B)</span><span style="color: black;">肿瘤中</span><span style="color: black;">SUVmax</span><span style="color: black;">的比较。</span><span style="color: black;">(C)</span><span style="color: black;">肿瘤与肌肉的</span><span style="color: black;">SUVmax</span><span style="color: black;">比值。</span><span style="color: black;">(D)</span><span style="color: black;">肿瘤组织离体</span><span style="color: black;">PET</span><span style="color: black;"><span style="color: black;">影像</span>。</span><span style="color: black;">(E)</span><span style="color: black;">肿瘤组织</span><span style="color: black;">% ID/g</span><span style="color: black;">的比较。</span><span style="color: black;">(F)</span><span style="color: black;">肿瘤 </span><span style="color: black;">SUVmax</span><span style="color: black;">与</span><span style="color: black;">rhACE2</span><span style="color: black;">剂量<span style="color: black;">关联</span>性分析。</span><span style="color: black;">(G)</span><span style="color: black;">肿瘤组织中</span><span style="color: black;">rhACE2</span><span style="color: black;">的</span><span style="color: black;">Western blot</span><span style="color: black;">。</span></span></p>
    <p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><strong style="color: blue;"><span style="color: black;">4<span style="color: black;">．</span></span><span style="color: black;">68</span><span style="color: black;">Ga‐HZ20<span style="color: black;">在</span><span style="color: black;">rhACE2</span><span style="color: black;">肝脏原位模型中的显像<span style="color: black;">科研</span></span></span></strong></p>
    <p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;"><span style="color: black;">为了<span style="color: black;">科研</span></span></span><span style="color: black;">68</span><span style="color: black;">Ga‐HZ20<span style="color: black;">探针对器官中</span><span style="color: black;">rhACE2</span><span style="color: black;">的</span></span><span style="color: black;"><span style="color: black;">检出</span></span><span style="color: black;"><span style="color: black;">能力，在</span>KM<span style="color: black;">小鼠肝脏原位注射<span style="color: black;">区别</span>剂量的</span><span style="color: black;">rhACE2</span><span style="color: black;">后进行了</span><span style="color: black;">PET</span><span style="color: black;"><span style="color: black;">影像</span><span style="color: black;">科研</span>。</span><span style="color: black;">PET</span></span><span style="color: black;"><span style="color: black;">图像</span></span><span style="color: black;"><span style="color: black;"><span style="color: black;">表示</span>探针在肝脏中有<span style="color: black;">显著</span>的</span>rhACE2<span style="color: black;">剂量依赖性摄取</span><span style="color: black;">(</span><span style="color: black;">图</span><span style="color: black;">4A)</span><span style="color: black;">。与对照组相比，</span><span style="color: black;">rhACE2</span><span style="color: black;">注射组肝脏</span><span style="color: black;">SUVmax</span><span style="color: black;">值更高，注射</span><span style="color: black;">15 min</span><span style="color: black;">后的</span><span style="color: black;">SUVmax</span><span style="color: black;">值分别为</span><span style="color: black;">0.43±0.02</span><span style="color: black;">、</span><span style="color: black;">0.52±0.02</span><span style="color: black;">和</span><span style="color: black;">0.76±0.01(</span><span style="color: black;">图</span><span style="color: black;">4B)</span><span style="color: black;">。</span><span style="color: black;">rhACE2</span><span style="color: black;">注射组靶</span></span><span style="color: black;"><span style="color: black;">本</span></span><span style="color: black;"><span style="color: black;">比更高，在</span>60 min<span style="color: black;">时分别为</span><span style="color: black;">1.3±0.20</span><span style="color: black;">、</span><span style="color: black;">2.54±0.27</span><span style="color: black;">、</span><span style="color: black;">3.95±0.52 (</span><span style="color: black;">图</span><span style="color: black;">4C)</span><span style="color: black;">。肝脏中</span><span style="color: black;">% ID/g</span><span style="color: black;"><span style="color: black;">亦</span>呈</span><span style="color: black;">rhACE2</span><span style="color: black;">剂量依赖性</span><span style="color: black;">(</span><span style="color: black;">图</span><span style="color: black;">4D)</span><span style="color: black;">。探针注射后</span><span style="color: black;">60 min rhACE2</span><span style="color: black;">与</span><span style="color: black;">SUVmax</span><span style="color: black;">的<span style="color: black;">关联</span>性最高，</span><span style="color: black;">R</span></span><span style="color: black;">2</span><span style="color: black;"><span style="color: black;">为</span>0.9994(<span style="color: black;">图</span><span style="color: black;">4E)</span><span style="color: black;">。</span><span style="color: black;">Western blotting</span><span style="color: black;">结果<span style="color: black;">表示</span></span><span style="color: black;">rhACE2</span><span style="color: black;">被成功注射到小鼠肝脏 </span><span style="color: black;">(</span><span style="color: black;">图</span><span style="color: black;">4F)</span><span style="color: black;">。</span></span></p>
    <p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><img src="https://mmbiz.qpic.cn/sz_mmbiz_png/Sdlx2O7ibpVnjQ4splicZicMhQuTuSWJ8KUyVt1YkN4AKOnUZuayk5NETTBpib2icjKfDNuWPES5hgw2guibpsnq8IIg/640?wx_fmt=png&amp;from=appmsg&amp;tp=webp&amp;wxfrom=5&amp;wx_lazy=1&amp;wx_co=1" style="width: 50%; margin-bottom: 20px;"></p>
    <p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;"><span style="color: black;">图</span>4. (A)<span style="color: black;">肝脏原位注射<span style="color: black;">区别</span>剂量</span><span style="color: black;">rhACE2</span><span style="color: black;">后的</span><span style="color: black;">PET</span><span style="color: black;"><span style="color: black;">影像</span>。白色圆圈<span style="color: black;">暗示</span>肝脏注射部位</span><span style="color: black;">(L</span><span style="color: black;">、</span><span style="color: black;">H</span><span style="color: black;">、</span><span style="color: black;">B</span><span style="color: black;">分别<span style="color: black;">表率</span>肝脏、心脏和膀胱</span><span style="color: black;">)</span><span style="color: black;">。</span><span style="color: black;">(B)</span><span style="color: black;">肝脏中</span><span style="color: black;">SUVmax</span><span style="color: black;">的比较。</span><span style="color: black;">(C)</span><span style="color: black;">肝脏与肌肉的</span><span style="color: black;">SUVmax</span><span style="color: black;">比值。</span><span style="color: black;">(D) </span><span style="color: black;"><span style="color: black;">区别</span>剂量组肝脏</span><span style="color: black;">% ID/g</span><span style="color: black;">比较。</span><span style="color: black;">(E) </span><span style="color: black;">肝脏</span><span style="color: black;">SUVmax</span><span style="color: black;">与</span><span style="color: black;">rhACE2</span><span style="color: black;">剂量的<span style="color: black;">关联</span>性分析。</span><span style="color: black;">(F)</span><span style="color: black;">肝脏中</span><span style="color: black;">rhACE2</span><span style="color: black;">的</span><span style="color: black;">Western blot</span><span style="color: black;">。</span></span></p>
    <p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><strong style="color: blue;"><span style="color: black;">5<span style="color: black;">．</span></span><span style="color: black;">68</span><span style="color: black;">Ga‐HZ20<span style="color: black;">在</span><span style="color: black;">rhACE2</span><span style="color: black;">脾脏原位模型中的显像<span style="color: black;">科研</span></span></span></strong></p>
    <p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;"><span style="color: black;">&nbsp; &nbsp; 将</span>rhACE2<span style="color: black;">原位注射到小鼠脾脏，</span><span style="color: black;">PET</span><span style="color: black;"><span style="color: black;">影像</span><span style="color: black;">表示</span></span><span style="color: black;">0.4 nmol</span><span style="color: black;">组探针<span style="color: black;">显著</span>聚集</span><span style="color: black;">(</span><span style="color: black;">图</span><span style="color: black;">5A)</span><span style="color: black;">。对照组与</span><span style="color: black;">0.2 nmol rhACE2</span><span style="color: black;">注射组脾脏摄取</span></span><span style="color: black;"><span style="color: black;">值</span></span><span style="color: black;"><span style="color: black;">差异较小，而</span>0.4 nmol<span style="color: black;">组<span style="color: black;">显著</span>高于其他两组，注射后</span><span style="color: black;">15 min</span><span style="color: black;">的</span><span style="color: black;">SUVmax</span><span style="color: black;">值分别为</span><span style="color: black;">0.28±0.03</span><span style="color: black;">、</span><span style="color: black;">0.28±0.01</span><span style="color: black;">和</span><span style="color: black;">0.58±0.01(</span><span style="color: black;">图</span><span style="color: black;">5B)</span><span style="color: black;">。<span style="color: black;">一样</span>，对照组和</span><span style="color: black;">0.2 nmol</span><span style="color: black;">组脾脏与肌肉的摄取比值<span style="color: black;">显著</span><span style="color: black;">小于</span></span><span style="color: black;">0.4 nmol</span><span style="color: black;">组，</span><span style="color: black;">60 min</span><span style="color: black;">分别为</span><span style="color: black;">1.25±0.20</span><span style="color: black;">、</span><span style="color: black;">1.30±0.14</span><span style="color: black;">和</span><span style="color: black;">4.13±0.05(</span><span style="color: black;">图</span><span style="color: black;">5C)</span><span style="color: black;">。离体显像结果<span style="color: black;">表示</span>出脾脏的<span style="color: black;">显著</span>摄取，尤其在</span><span style="color: black;">rhACE2</span><span style="color: black;">注射点更加浓聚</span><span style="color: black;">(</span><span style="color: black;">图</span><span style="color: black;">5D)</span><span style="color: black;">。</span><span style="color: black;">rhACE2</span><span style="color: black;">注射组的</span><span style="color: black;">% ID/g</span><span style="color: black;"><span style="color: black;">明显</span>高于对照组，分别为</span><span style="color: black;">0.41±0.01</span><span style="color: black;">、</span><span style="color: black;">0.53±0.01</span><span style="color: black;">和</span><span style="color: black;">0.86±0.03(</span><span style="color: black;">图</span><span style="color: black;">5E)</span><span style="color: black;">。探针注射后</span><span style="color: black;">45 min</span><span style="color: black;">时</span><span style="color: black;">rhACE2</span><span style="color: black;">与</span><span style="color: black;">SUVmax</span><span style="color: black;">的相关性最高，</span><span style="color: black;">R</span></span><span style="color: black;">2</span><span style="color: black;"><span style="color: black;">为</span>0.9095(<span style="color: black;">图</span><span style="color: black;">5F)</span><span style="color: black;">。<span style="color: black;">一样</span>，</span><span style="color: black;">Western blot</span><span style="color: black;">结果<span style="color: black;">表示</span>脾脏中几乎<span style="color: black;">无</span></span><span style="color: black;">ACE2</span><span style="color: black;">的表达， </span><span style="color: black;">rhACE2</span><span style="color: black;">注射组中观察到</span><span style="color: black;">rhACE2</span><span style="color: black;">条带，但其含量<span style="color: black;">显著</span><span style="color: black;">小于</span>相同剂量的其他模型组，分析其<span style="color: black;">原由</span>可能是<span style="color: black;">因为</span>脾脏体积小，术中</span><span style="color: black;">rhACE2</span><span style="color: black;">渗漏<span style="color: black;">导致</span></span><span style="color: black;">(</span><span style="color: black;">图</span><span style="color: black;">5G)</span><span style="color: black;">。</span></span></p>
    <p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><img src="https://mmbiz.qpic.cn/sz_mmbiz_png/Sdlx2O7ibpVnjQ4splicZicMhQuTuSWJ8KUpicUeAIBgRuS9P9chXpJjMhHTUY2dCWYyJnibdhQx2ibiciasHNibD4iaDCaQ/640?wx_fmt=png&amp;from=appmsg&amp;tp=webp&amp;wxfrom=5&amp;wx_lazy=1&amp;wx_co=1" style="width: 50%; margin-bottom: 20px;"></p>
    <p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;"><span style="color: black;">图</span>5. (A)<span style="color: black;">脾脏原位注射<span style="color: black;">区别</span>剂量</span><span style="color: black;">rhACE2</span><span style="color: black;">的</span><span style="color: black;">PET/CT</span><span style="color: black;"><span style="color: black;">影像</span>。白色方框为脾脏 </span><span style="color: black;">(K</span><span style="color: black;">、</span><span style="color: black;">B</span><span style="color: black;">分别<span style="color: black;">表率</span>肾脏和膀胱</span><span style="color: black;">)</span><span style="color: black;">。</span><span style="color: black;">(B)</span><span style="color: black;">脾脏中</span><span style="color: black;">SUVmax</span><span style="color: black;">的比较。</span><span style="color: black;">(C)</span><span style="color: black;">脾脏与肌肉的</span><span style="color: black;">SUVmax</span><span style="color: black;">之比。</span><span style="color: black;">(D)</span><span style="color: black;">脾脏离体</span><span style="color: black;">PET</span><span style="color: black;">显像。</span><span style="color: black;">(E)</span><span style="color: black;"><span style="color: black;">区别</span>剂量组脾脏</span><span style="color: black;">% ID/g</span><span style="color: black;">比较。</span><span style="color: black;">(F) </span><span style="color: black;">脾脏</span><span style="color: black;">SUVmax</span><span style="color: black;">与</span><span style="color: black;">rhACE2</span><span style="color: black;">剂量的<span style="color: black;">关联</span>性分析。</span><span style="color: black;">(G)</span><span style="color: black;">脾脏中</span><span style="color: black;">rhACE2</span><span style="color: black;">的</span><span style="color: black;">Western blot</span><span style="color: black;">。</span></span></p>
    <p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><strong style="color: blue;"><span style="color: black;">6<span style="color: black;">．</span></span><span style="color: black;">68</span><span style="color: black;">Ga‐HZ20<span style="color: black;">在</span><span style="color: black;">rhACE2-</span><span style="color: black;">脑原位模型中的显像<span style="color: black;">科研</span></span></span></strong></p>
    <p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;">&nbsp; &nbsp; Micro-PET<span style="color: black;"><span style="color: black;">影像</span><span style="color: black;">表示</span>，探针在对照组小鼠脑内有轻微摄取，</span></span><span style="color: black;"><span style="color: black;">在</span></span><span style="color: black;">rhACE2<span style="color: black;">注射组脑内更加<span style="color: black;">显著</span></span><span style="color: black;">(</span><span style="color: black;">图</span><span style="color: black;">6A)</span><span style="color: black;">。</span><span style="color: black;">SUVmax</span><span style="color: black;">分析<span style="color: black;">表示</span>，探针注射后任一时间点，</span><span style="color: black;">rhACE2</span><span style="color: black;">注射组与对照组的摄取值均有统计学<span style="color: black;">道理</span> </span><span style="color: black;">(</span><span style="color: black;">图</span><span style="color: black;">6B)</span><span style="color: black;">。脑与肌肉的靶本比<span style="color: black;">亦</span><span style="color: black;">表示</span>出一致的结果，</span><span style="color: black;">120 min</span><span style="color: black;">时分别为</span><span style="color: black;">2.23±0.10</span><span style="color: black;">、</span><span style="color: black;">2.85±0.06</span><span style="color: black;">、</span><span style="color: black;">3.34±0.12</span><span style="color: black;">和</span><span style="color: black;">4.23±0.12(</span><span style="color: black;">图</span><span style="color: black;">6C)</span><span style="color: black;">。脑组织离体显像<span style="color: black;">表示</span>注射组脑中有<span style="color: black;">显著</span>的探针摄取，<span style="color: black;">尤其</span>是</span><span style="color: black;">0.8 nmol</span><span style="color: black;">组</span><span style="color: black;">(</span><span style="color: black;">图</span><span style="color: black;">6D)</span><span style="color: black;">。脑组织</span><span style="color: black;">% ID/g</span><span style="color: black;"><span style="color: black;">亦</span><span style="color: black;">表示</span></span><span style="color: black;">rhACE2</span><span style="color: black;">注射组<span style="color: black;">明显</span>高于对照组</span></span><span style="color: black;">(</span><span style="color: black;"><span style="color: black;">图</span>6E</span><span style="color: black;">)</span><span style="color: black;"><span style="color: black;">。探针注射后</span>30 min rhACE2<span style="color: black;">与</span><span style="color: black;">SUVmax</span><span style="color: black;">的<span style="color: black;">关联</span>性最高，</span><span style="color: black;">R</span></span><span style="color: black;">2</span><span style="color: black;"><span style="color: black;">为</span>0.9913(<span style="color: black;">图</span><span style="color: black;">6F)</span><span style="color: black;">。</span><span style="color: black;">Western blot</span><span style="color: black;"><span style="color: black;">表示</span>脑组织本身有微量</span><span style="color: black;">ACE2</span><span style="color: black;">表达，注射组有<span style="color: black;">显著</span>聚集 </span><span style="color: black;">(</span><span style="color: black;">图</span><span style="color: black;">6G)</span><span style="color: black;">。</span></span></p>
    <p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><img src="https://mmbiz.qpic.cn/sz_mmbiz_png/Sdlx2O7ibpVnjQ4splicZicMhQuTuSWJ8KU4zyxJwbl07XmONH6TWpuIVnCoWdfFAeWwDbodlUmefYsk9XibUlEia9w/640?wx_fmt=png&amp;from=appmsg&amp;tp=webp&amp;wxfrom=5&amp;wx_lazy=1&amp;wx_co=1" style="width: 50%; margin-bottom: 20px;"></p>
    <p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;"><span style="color: black;">图</span>6. (A)<span style="color: black;">脑内原位注射<span style="color: black;">区别</span>剂量</span><span style="color: black;">rhACE2</span><span style="color: black;">的</span><span style="color: black;">PET/CT</span><span style="color: black;"><span style="color: black;">影像</span>。白色圆圈<span style="color: black;">暗示</span>脑注射部位</span><span style="color: black;">(K, B</span><span style="color: black;">分别<span style="color: black;">表率</span>肾脏和膀胱</span><span style="color: black;">)</span><span style="color: black;">。</span><span style="color: black;">(B)</span><span style="color: black;">脑内</span><span style="color: black;">SUVmax</span><span style="color: black;">的比较。</span><span style="color: black;">(C)</span><span style="color: black;">脑与肌肉</span><span style="color: black;">SUVmax</span><span style="color: black;">的比值。</span><span style="color: black;">(D)</span><span style="color: black;">脑组织离体</span><span style="color: black;">PET</span><span style="color: black;">显像。</span><span style="color: black;">(E)</span><span style="color: black;">脑组织</span><span style="color: black;">% ID/g</span><span style="color: black;">比较。</span><span style="color: black;">(F) </span><span style="color: black;">脑</span><span style="color: black;">SUVmax</span><span style="color: black;">与</span><span style="color: black;">rhACE2</span><span style="color: black;">剂量的<span style="color: black;">关联</span>性分析。</span><span style="color: black;">(G)</span><span style="color: black;">脑中</span><span style="color: black;">rhACE2</span><span style="color: black;">的</span><span style="color: black;">Western blot</span><span style="color: black;">。</span></span></p>
    <p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;"><strong style="color: blue;"><span style="color: black;">小结与展望：</span></strong></span></p>
    <p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;">1.</span><span style="color: black;"><span style="color: black;">本<span style="color: black;">科研</span><span style="color: black;">经过</span>对标记<span style="color: black;">要求</span>的优化，制备了高放化产率的</span>ACE2<span style="color: black;">靶向核素探针</span></span><span style="color: black;">68</span><span style="color: black;">Ga‐HZ20<span style="color: black;">。</span></span></p>
    <p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;">2.</span><span style="color: black;"><span style="color: black;"><span style="color: black;">经过</span><span style="color: black;">运用</span></span></span><span style="color: black;">68</span><span style="color: black;">Ga-HZ20<span style="color: black;">探针对小鼠</span>“rhACE2-<span style="color: black;">组织</span>/<span style="color: black;">器官</span>”<span style="color: black;">原位模型进行</span>PET/CT<span style="color: black;">显像，<span style="color: black;">创立</span>了探针摄取与</span>rhACE2<span style="color: black;">剂量高度<span style="color: black;">关联</span>性，有助于</span>rhACE2<span style="color: black;">疗法的进一步<span style="color: black;">科研</span>和应用。</span></span></p>
    <p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;">3.</span><span style="color: black;"><span style="color: black;">首次成功构建了</span>rhACE2-<span style="color: black;">组织</span>/<span style="color: black;">器官原位模型，<span style="color: black;">针对</span>其他模型的构建及探针的快速<span style="color: black;">评估</span><span style="color: black;">供给</span>了思路。</span></span></p>
    <p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;">//<strong style="color: blue;"><span style="color: black;"><span style="color: black;">通讯作者</span></span></strong><span style="color: black;"><span style="color: black;">为西南医科大学</span></span><strong style="color: blue;"><span style="color: black;"><span style="color: black;">刘友平</span></span></strong><span style="color: black;"><span style="color: black;">教授、北京大学肿瘤医院</span></span><strong style="color: blue;"><span style="color: black;"><span style="color: black;">朱华</span></span></strong><span style="color: black;"><span style="color: black;"><span style="color: black;">科研</span>员、北京大学深圳<span style="color: black;">科研</span>生院</span></span><strong style="color: blue;"><span style="color: black;"><span style="color: black;">刘琦</span></span></strong><span style="color: black;"><span style="color: black;"><span style="color: black;">科研</span>员。</span></span></span></p>
    <p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><strong style="color: blue;"><span style="color: black;"><span style="color: black;"><span style="color: black;">第1</span>作者</span></span></strong><span style="color: black;"><span style="color: black;">为北京大学肿瘤医院与西南医科大学联合培养的</span></span><strong style="color: blue;"><span style="color: black;"><span style="color: black;">王紫蕾</span></span></strong><span style="color: black;"><span style="color: black;"><span style="color: black;">朋友</span>、北京大学肿瘤医院</span></span><strong style="color: blue;"><span style="color: black;"><span style="color: black;">赵传科</span></span></strong><span style="color: black;"><span style="color: black;">副<span style="color: black;">科研</span>员、河北北方学院<span style="color: black;">附庸</span><span style="color: black;">第1</span>医院</span></span><strong style="color: blue;"><span style="color: black;"><span style="color: black;">李传贵</span></span></strong><span style="color: black;"><span style="color: black;">专家</span>医师</span><span style="color: black;"><span style="color: black;">。</span></span><span style="color: black;">//</span></p>
    <p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;">Author information</span></p>
    <p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><strong style="color: blue;"><span style="color: black;"><span style="color: black;">原文链接</span></span><span style="color: black;"><span style="color: black;">：</span></span></strong><span style="color: black;"><span style="color: black;">http://doi.org/10.1002/jmv.29221</span></span></p>
    <p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;">Citation:</span><span style="color: black;">Wang, Z., Zhao, C., Li, C., Liu, S., Ding, J., He, C., Liu, J., Dong, B., Yang, Z., Liu, Q., Zhu, H., &amp; Liu, Y. (2023). Molecular PET/CT mapping of rhACE2 distribution and quantification in organs to aid in SARS-CoV-2 targeted therapy. Journal of medical virology, 95(11), e29221. https://doi.org/10.1002/jmv.29221.</span></p>




1fy07h 发表于 2024-10-13 00:34:59

楼主发的这篇帖子，我觉得非常有道理。
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